What is Dulbecco's Modification of Eagle's Medium (DMEM) used for?

Dulbecco's Modification of Eagle's Medium (DMEM) is primarily used for culturing mammalian cells in laboratories. This medium provides essential nutrients, such as glucose and sodium pyruvate, supporting the growth and maintenance of diverse cell lines. It’s particularly beneficial for studies in cellular biology, drug development, and biotechnology. For example, researchers can use DMEM to assess cellular responses to treatment or to explore fundamental cellular processes.

Why is L-glutamine excluded in this formulation?

This formulation of DMEM deliberately excludes L-glutamine, making it suitable for applications where glutamine supplementation can lead to media instability or toxicity. Some cell types benefit from using other forms of nitrogen sources or are better suited to glutamine-free environments, allowing researchers to tailor the medium to specific cell line needs. By excluding L-glutamine, you can reduce variables in experimental conditions and prolong the medium's shelf life.

How do I prepare the Mediatech 15-013-LX DMEM powder?

To prepare the DMEM powder, start by reconstituting it with sterile water according to the instructions provided. Generally, dissolve the required amount of powder in the appropriate volume of sterile water to achieve the desired concentration. After dissolving, it's important to adjust the pH if necessary and filter through a 0.22 µm filter to ensure sterility. This preparation process is crucial for maintaining the viability and health of cultured cells.

What types of cell lines are compatible with this medium?

Mediatech 15-013-LX DMEM is compatible with a wide range of mammalian cell lines, including fibroblasts, epithelial, and various cancer cell lines. This versatility makes it suitable for applications in basic research, as well as in development processes for vaccines or therapies. Notably, researchers often employ it for projects involving human cells, such as stem cells or primary cell cultures, due to its rich nutrient profile.

Can this medium be supplemented with other components?

Yes, this medium can be supplemented with various components based on specific experimental requirements. Common supplements include fetal bovine serum (FBS) for nutrient enrichment, or antibiotics to prevent contamination. Additionally, researchers may add growth factors or hormones depending on the cell lines being cultured. Customizing the medium in this way allows for optimized growth conditions tailored to specific research goals.

What are the storage conditions for the Mediatech DMEM powder?

To ensure the longevity and efficacy of the DMEM powder, it should be stored in a cool, dry place, preferably at 2-8°C. Once reconstituted, unused medium should typically be stored in the refrigerator and is generally used within a few days, depending on laboratory standards and cell culture practices. Proper storage is key to maintaining the quality and integrity of the medium for reliable experimental results.

What is the significance of glucose in the DMEM formula?

Glucose serves as a primary energy source for cells, pivotal for maintaining cellular metabolism and supporting growth. In the Mediatech DMEM formula, the concentration of 4.5 g/L ensures that cultured cells receive adequate energy for vital functions such as protein synthesis and proliferation. For applications in metabolic studies or drug responses, the presence of glucose is crucial to mimic physiological conditions.

How is sodium pyruvate beneficial in this medium?

Sodium pyruvate acts as an important substrate for energy production and plays a role in cellular metabolism. Its inclusion in the DMEM formulation provides an alternative energy source, supporting cell viability, particularly in cases of glucose deprivation or metabolic stress. Researchers utilize sodium pyruvate to enhance the overall media effectiveness for challenging cell lines or diverse research applications, promoting optimal cell health.

What precautions should be taken when using DMEM in experiments?

When using DMEM for cell culture, it’s important to maintain sterile techniques to avoid contamination. This includes using sterile equipment, avoiding direct contact with the media, and working within a laminar flow hood. Additionally, regularly monitor cell health and medium conditions, as changes in pH or turbidity can indicate contamination or nutrient depletion. Precautionary measures ensure the reliability of experimental outcomes.

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Dulbecco's Modified Eagle's Medium supports a variety of normal and transformed cells, making it essential for diverse cell culture applications.

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Item #: 188068541

Mediatech 15-013-LX Dulbecco's Modification of Eagle's Medium, Powder, 4.5 g/L Glucose and Sodium Pyruvate Without L-glutamine, 10 lb.

Q: Where can I buy Mediatech 15-013-LX Dulbecco's Modification Of Eagle's Medium in Ireland?

You can purchase Mediatech 15-013-LX Dulbecco's Modification Of Eagle's Medium exclusively at Ubuy in Ireland. Ubuy offers an easy online shopping experience, ensuring you can find this specific medium along with other essential laboratory supplies. The platform often provides additional details about the products and customer service support to help guide you in your purchase.

Item #: 188068541

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Dulbecco's Modified Eagle's Medium supports a variety of normal and transformed cells, making it essential for diverse cell culture applications.

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What Stands Out

High Glucose Content

Contains 4.5 g/L glucose, providing optimal energy for cell growth and enhancing cellular metabolism in various applications.

Customizable Formula

Designed without L-glutamine, allowing researchers to tailor media components based on specific experimental requirements and cell line needs.

Bulk Packaging

10 lb bulk size ensures cost-effectiveness and convenience for laboratories, minimizing frequent reordering and ensuring a steady supply for ongoing experiments.

Product Details

Container Type	Bag
Item Form	Powder
Item Weight	10 pounds (4.54 kg)
Brand Name	Mediatech
Unit Count	1 Count
Manufacturer	Mediatech, Inc., a Corning Subsidiary
Model Number	15-013-LX
Part Number	15-013-LX
Brand	Mediatech
Package Information	Bag

Who Should Buy?

Cell Culture Researchers
Ideal for researchers in cellular biology needing a reliable medium for various cell types in culture.
Pharmaceutical Development
Suitable for drug testing and development processes requiring specific nutrient formulations for cell viability.
Educational Institutions
Beneficial for universities and colleges teaching biology labs focusing on cell culture techniques and applications.

Neuroscience Studies
Not ideal for neural cells which might require specialized media for optimal growth and maintenance.

Product Description

Mediatech 15-013-LX Dulbecco's Modification of Eagle's Medium, Powder, 4.5 g/L Glucose and Sodium Pyruvate Without L-glutamine, 10 lb.

Dietary Supplement Disclaimer

Statements regarding dietary supplements have not been evaluated by the Food and Drug Administration and are not intended to diagnose, treat, cure, or prevent any disease or health condition.

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Customer Questions & Answers

Question: How to Shop Mediatech 15-013-LX Dulbecco's Modification of Online From Ubuy?

Answer: It’s easy to shop Mediatech 15-013-LX Dulbecco's Modification of online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is Mediatech 15-013-LX Dulbecco's Modification of Available to Shop Online in Ireland?

Answer: Yes, at Ubuy Ireland this product is available for you to shop at a reasonable price. The Mediatech 15-013-LX Dulbecco's Modification of is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

Nutritionist's Take

Mediatech's Dulbecco's Modification of Eagle's Medium (DMEM) is primarily a cell culture medium rather than a direct nutritional product for human consumption. It contains 4.5 g/L of glucose and sodium pyruvate, which are crucial for cellular energy metabolism and maintaining pH balance in cell culture systems. While it may provide certain nutrients conducive to cell growth, this product is not designed for dietary use in humans and lacks essential components like L-glutamine, which supports cell proliferation. Therefore, nutritionists advise against its consumption as a food product for humans as it does not provide balanced nutrition or fit typical dietary needs. It is suitable for laboratory research rather than a healthy choice for individual nutrition.

Mediatech Sodium 15-013-LX Editorial Review

Mediatech 15-013-LX Dulbecco's Modification of Eagle's Medium is a versatile and essential powder designed for cell culture applications. With a significant formulation containing 4.5 g/L glucose and sodium pyruvate, this 10 lb. bag is ideal for researchers and laboratories needing reliable media. Users appreciate its easy mixture and effectiveness for growing various cell lines, ensuring optimal growth conditions. This medium does not contain L-glutamine, allowing for more control over the growth factors added later. Mediatech, a trusted name in the industry, offers this product, ensuring high-quality standards in laboratory settings.

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Pros

Easy to mix and use in labs
Supports various cell culture applications
High quality from a trusted brand
Contains essential glucose and sodium pyruvate
Available in a convenient bag packaging

Cons

Some users may prefer a pre-made solution instead of powder

Product Price History

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€ 746

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Features & Benefits

Supports a wide range of normal and transformed cells.
Enhanced formulation with four-fold concentrations of amino acids and vitamins.
Ideal for culturing embryonic mouse and chicken cells.
High-quality medium developed for robust cell growth.
Package dimensions: 6 L x 12 H x 14 W inches.
Weight: 24 pounds, made in the USA.

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Mediatech 15-013-LX Dulbecco's Modification of Eagle's Medium, Powder, 4.5 g/L Glucose and Sodium Pyruvate Without L-glutamine, 10 lb.

€ 746

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100% Original Products

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What Stands Out

Product Details

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Product Description

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Nutritionist's Take

Mediatech Sodium 15-013-LX Editorial Review

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Important information

€ 746

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