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![TBS [10X]; Tris buffered Saline 100mM Tris.HCl, 1.5M NaCl, pH 7.5 - 500mL](https://m.media-amazon.com/images/I/31Hra4HFjcL._SS400_.jpg)
Item #:
97963801
TBS [10X]; Tris buffered Saline 100mM Tris.HCl, 1.5M NaCl, pH 7.5 - 500mL
Item #:
97963801
€ 64
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What Stands Out
Optimal pH
Designed to maintain a stable pH of 7.5, ensuring optimal conditions for biochemical reactions and experiments.
High Concentration
Contains 1.5M NaCl, providing a physiological salt concentration ideal for enhancing protein solubility and stability.
Ready-to-use
Comes in a convenient 500mL bottle, pre-mixed for easy application, saving time and reducing preparation errors.
Product Details
| Item Weight | 1.1 lbs (500 grams) |
Who Should Buy?
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Laboratory Research
Ideal for researchers conducting biological and biochemical experiments requiring a stable pH buffer system.
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Cell Culture
Suitable for maintaining osmotic balance and pH in cell culture applications, enhancing cell viability.
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Molecular Biology
Useful in molecular biology techniques, such as DNA and protein electrophoresis, ensuring consistent buffer conditions.
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Field Work
Not ideal for outdoor experiments due to stability issues and potential contamination in uncontrolled environments.
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Non-Biological Uses
Inappropriate for chemical reactions or applications outside of biological or biochemical scientific research.
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Beginner's Use
May not be suitable for beginners without proper training in handling reagents and conducting experiments.
Product Description
TBS [10X]; Tris buffered Saline 100mM Tris.HCl, 1.5M NaCl, pH 7.5 - 500mL
Have any Query? Chat with us
Customer Questions & Answers
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Question:
What is TBS [10X]; Tris buffered Saline used for?
Answer: TBS [10X]; Tris buffered Saline is primarily used as a diluent and buffer solution in various biochemical applications, including protein and nucleic acid research. This formulation ensures a stable pH environment, which is essential for maintaining the functionality of biomolecules. Common scenarios include Western blotting, ELISA, and immunohistochemistry where pH stability is crucial for accurate results. -
Question:
What is the composition of TBS [10X]; Tris buffered Saline?
Answer: The TBS [10X] consists of 100 mM Tris.HCl and 1.5 M NaCl, adjusted to a pH of 7.5. Tris is a widely used buffering agent that helps to maintain the stability of the pH in biological experiments, while NaCl provides the necessary ionic strength. This specific formulation is designed for easy dilution to a working concentration, making it versatile for laboratory use. -
Question:
How do I prepare a working solution from TBS [10X]; Tris buffered Saline?
Answer: To prepare a working solution from TBS [10X], simply dilute the stock solution with distilled water. For a 1X solution, mix one part of TBS 10X with nine parts of distilled water. Thorough mixing is essential to ensure that the buffering capacity is uniform. This dilution is commonly used in various experiments where a buffered saline solution is required. -
Question:
Is TBS [10X]; Tris buffered Saline suitable for cell culture?
Answer: While TBS [10X] is not specifically designed for cell culture, it can be used for washing cells or diluting antibodies in immunological assays. However, for cell culture maintenance, other media like DMEM or RPMI are preferable. When using TBS in conjunction with cell culture, ensure that it does not interfere with cell viability or experimental outcomes. -
Question:
What should I store TBS [10X]; Tris buffered Saline?
Answer: TBS [10X] should be stored at 2-8°C for optimal stability. Avoid repeated freezing and thawing to prevent degradation of the components. Proper storage helps maintain the integrity of the solution over time, making it ready for subsequent experiments without significant loss in quality or performance. -
Question:
Can I use TBS [10X]; Tris buffered Saline for antibody dilution?
Answer: Yes, TBS [10X] is commonly used for diluting antibodies in various immunological assays. The buffered environment provides a suitable pH for maintaining antibody stability and activity, ensuring optimal results in applications such as Western blotting and immunofluorescence. Always verify the compatibility of your antibodies with the buffer used. -
Question:
What are the advantages of using TBS [10X]; Tris buffered Saline in experiments?
Answer: The main advantages of using TBS [10X] include its ability to maintain a stable pH and its isotonic properties, which help preserve the integrity of biomolecules. It is particularly beneficial in protocols requiring accurate pH control, such as during enzymatic reactions or when performing assays that are sensitive to ionic strength and buffering capacity. -
Question:
What is the pH level of TBS [10X]; Tris buffered Saline?
Answer: The pH level of TBS [10X] is precisely set at 7.5. This neutral pH is critical for many biochemical applications, allowing researchers to maintain protein and nucleic acid stability during experiments. The choice of pH 7.5 is optimal for a wide range of biological systems and reactions. -
Question:
What is the shelf life of TBS [10X]; Tris buffered Saline?
Answer: TBS [10X] typically has a long shelf life when stored under recommended conditions. It may remain usable for several months to years depending on storage practices and absence of contaminants. It is always advisable to visually inspect the solution for any signs of degradation or precipitation before use. -
Question:
Where can I buy TBS [10X]; Tris buffered Saline 100mM Tris.HCl, 1.5M NaCl, pH 7.5 - 500mL?
Answer: You can purchase TBS [10X]; Tris buffered Saline from Ubuy. They offer a wide range of laboratory reagents including this buffer solution, making it convenient for researchers and lab personnel in Ireland. Visit their website for more information on product availability and ordering options.
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€ 64
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Features & Benefits
- Comprehensive range of buffers for research and diagnostics.
- Essential for protein research, molecular biology, and characterization.
- Produced in an ISO 9001 and 13485 certified facility.
- Manufactured under stringent Good Manufacturing Practices.
- Quality control tests conducted in certified 14644 clean room conditions.
- Ideal for ELISA, Western blotting, and other immunoassays.
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