What is 10X PBS and what are its primary components?

10X PBS, or Phosphate Buffered Saline, is a versatile buffer solution commonly used in biological research. Its primary components include sodium chloride, potassium phosphate, and sodium phosphate, which help maintain a constant pH of 7.4. PBS is essential for maintaining osmotic balance and nutrient availability in various biological applications, such as cell culture, protein assays, and molecular biology techniques.

Is 10X PBS RNase/DNase free and why is this important?

Yes, this 10X PBS is RNase and DNase free, which is crucial for experiments involving RNA and DNA. Contamination with these enzymes can lead to degradation of nucleic acids, compromising experimental results. This makes RNase/DNase free PBS particularly suited for sensitive assays, including qPCR and nucleic acid purification where integrity is paramount.

What does the 0.22 µm filtration mean for 10X PBS?

The 0.22 µm filtration of 10X PBS ensures that the solution is free from bacteria and other potential contaminants. This high level of filtration is important in maintaining sterility, particularly for applications involving cell cultures or sensitive biochemical assays, as it reduces the risk of contamination that could affect experimental outcomes.

How do you dilute 10X PBS for typical experiments?

To dilute 10X PBS for most applications, mix one part of the 10X solution with nine parts of distilled water, resulting in 1X PBS. This dilution provides a balanced environment for cells and proteins in biological experiments. For example, if you require 100 mL of 1X PBS, you would mix 10 mL of 10X PBS with 90 mL of distilled water.

Can 10X PBS be used for cell culture?

Absolutely! 10X PBS is a common choice for cell culture applications. It helps maintain the physiological pH and osmotic balance of the environment where cells are grown, promoting healthy growth and functioning. PBS can be used for washing cells or diluting cell suspension before experiments, making it an essential tool in cell biology.

Is 10X PBS suitable for immunological assays?

Yes, 10X PBS is ideal for use in immunological assays, such as ELISA or Western blotting. Its balanced ions maintain the stability of proteins and antigens without denaturation. In these assays, PBS is often used for diluting antibodies or washing microplates, ensuring that the integrity and functionality of the proteins are preserved.

What safety measures should be taken when using 10X PBS?

While 10X PBS is generally safe to use, standard lab safety procedures should always be followed. This includes wearing gloves, lab coats, and eye protection to avoid any potential skin or eye irritation. Additionally, ensure that work is done in a sterile environment to maintain the integrity of your solutions and experiments.

Does 10X PBS have an expiration date?

Yes, 10X PBS does have an expiration date, which is typically provided by the manufacturer. It's important to use the solution before its expiration to ensure that its pH and ionic balance remains effective for experimental applications. Store PBS as per the manufacturer's recommendations, usually in a cool, dark place, to prolong its stability.

What are some common applications of 10X PBS in molecular biology?

10X PBS is widely used in molecular biology applications, including cell culture, protein purification, genetic studies, and various assays like ELISA and Western blotting. Its buffer capacity and unique composition make it an essential tool for maintaining cellular and biochemical assays, ensuring optimal conditions for reactions involving nucleic acids and proteins.

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Is 10X PBS (Phosphate Buffered Saline), pH 7.4 - Available to Shop Online in Ireland?

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UFC Bio

10X PBS (Phosphate Buffered Saline), pH 7.4 - Molecular Biology Grade - RNase/DNase Free, Sterile, 0.22um Filtered - 500 mL

UFC Bio

Item #: 60673543

10X PBS (Phosphate Buffered Saline), pH 7.4 - Molecular Biology Grade - RNase/DNase Free, Sterile, 0.22um Filtered - 500 mL

Q: Where can I buy 10X PBS Phosphate Buffered Saline, pH 7.4 - Molecular Biology Grade?

You can conveniently purchase 10X PBS Phosphate Buffered Saline, pH 7.4 - Molecular Biology Grade, from Ubuy in Ireland. Ubuy offers a wide selection of laboratory chemicals and reagents, including PCR mixtures, buffers, and sterile solutions, ensuring you find the product you need for your research.

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Item #: 60673543

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What Stands Out

Molecular Biology Grade

Optimized for laboratory use, ensuring high purity suitable for sensitive molecular biology applications, providing reliable results for researchers and scientists.

RNase/DNase Free

Guaranteed absence of RNase and DNase enzymes, protecting nucleic acid integrity during experiments, making it ideal for protocols requiring utmost DNA and RNA preservation.

Sterile and Filtered

Each 500 mL bottle is sterilized and 0.22um filtered, preventing contamination and ensuring readiness for immediate use in critical experiments or assays.

Product Details

Package Weight

1.18 Pound

Who Should Buy?

Molecular Biologists
Ideal for experiments requiring a stable pH and buffer system, aiding in the preservation of biological samples.
Cell Culture
Suitable for diluting cells and maintaining osmotic balance, essential for growing and propagating cell lines.
Protein Extraction
Effective for diluting or solubilizing proteins while preventing degradation, crucial for accurate analysis and experiments.

General Laboratory Use
Not ideal for applications outside of molecular biology where specific buffers aren’t required for experiments.

Product Description

10X PBS (Phosphate Buffered Saline), pH 7.4 - Molecular Biology Grade - RNase/DNase Free, Sterile, 0.22um Filtered - 500 mL

About This Item

If you're looking for a high-quality buffer solution for your laboratory, look no further than 10X PBS Phosphate Buffered Saline. Specifically designed for research purposes, this molecular biology grade PBS is guaranteed to be RNaseDNase free, sterile, and 0.22um filtered. Whether you're doing cell culture, protein chemistry, or molecular biology, 10X PBS Buffer is an essential component of your workflow. With a pH of 7.4, it's the perfect solution for a wide range of applications. But what sets this buffer apart is the fact that it's molecular biology grade.

That means it's been manufactured to the highest standards, ensuring that it meets the stringent requirements of researchers in the field. You can trust that your experiments will be accurate and reliable when you use 10X PBS Buffer. And with the added benefits of being RNaseDNase free and sterile filtered, you can be confident that your experiments won't be contaminated by unwanted biological agents. So whether you're an experienced researcher or just starting out in the lab, 10X PBS Buffer is the perfect choice for all your buffer needs. Don't ingest it though, as it's for research purposes only.

Order your 500 mL bottle today and experience the difference that molecular biology grade 10X PBS Phosphate Buffered Saline can make!.

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Product Buying Guide

10X PBS Buffer is a key reagent used in molecular biology research for various applications. This buying guide will provide you with essential information to make an informed purchase decision based on the product specifications, key features, usage scenarios, comparison with competitors, user reviews, price analysis, and buying considerations. Continue Reading

Customer Questions & Answers

Question: How to Shop 10X PBS (Phosphate Buffered Saline), pH 7.4 - Online From Ubuy?

Answer: It’s easy to shop 10X PBS (Phosphate Buffered Saline), pH 7.4 - online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is 10X PBS (Phosphate Buffered Saline), pH 7.4 - Available to Shop Online in Ireland?

Answer: Yes, at Ubuy Ireland this product is available for you to shop at a reasonable price. The 10X PBS (Phosphate Buffered Saline), pH 7.4 - is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

UFC Bio Buffers Editorial Review

10X PBS (Phosphate Buffered Saline), pH 7.4 - Molecular Biology Grade - RNase/DNase Free, Sterile, 0.22um Filtered - 500 mL is a high-quality buffer solution essential for various molecular biology applications. Manufactured by UFC Bio, this sterile 10X PBS is supplied in a convenient 500 mL bottle, ensuring easy handling and storage. The formulation is designed to maintain a stable pH, providing optimal conditions for sample preparation and cell cultivation. Additionally, it is RNase and DNase free, making it suitable for sensitive experiments requiring contamination-free environments. Users appreciate its reliability and performance in diverse laboratory settings.

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Pros

Sterile and contamination-free for reliable results
Convenient 500 mL bottle for easy use
Maintains stable pH for optimal conditions
Perfect for molecular biology applications
RNase and DNase free formulation

Product Price History

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10X PBS (Phosphate Buffered Saline), pH 7.4 - Molecular Biology Grade - RNase/DNase Free, Sterile, 0.22um Filtered - 500 mL