ProSignalTM Femto ECL Reagent, Low-femtogram, 1000cm2, 100ml KitUnit
€ 573
Price Details
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ProSignal Pico has an intense signal that lasts 6+ hours after substrate incubation, offering flexible imaging and allowing multiple exposures to be carried out without substantial signal decay.
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Product Details
| Country of Origin | This item will be imported from US |
| Item Weight | 1.2 lbs (540 grams) |
| What is in the box | ProSignalTM Femto ECL Reagent... For more details, please check description/product details |
Who Should Buy?
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Biomedical Researchers
Ideal for researchers requiring sensitive detection of low-abundance proteins in complex biological samples using ECL techniques.
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Diagnostic Laboratories
Suitable for labs performing assays that necessitate low picogram sensitivity for precise biomarker detection.
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Pharmaceutical Companies
Beneficial for companies involved in drug development that need reliable quantification of proteins in their studies.
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General Hobbyists
Not suitable for hobbyists due to its specialized application and sensitivity, requiring professional technical expertise.
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High-Volume Screening
May not be effective for high-throughput screening where faster, less sensitive methods are preferred for bulk testing.
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Non-Biological Applications
Not appropriate for applications outside of biological research, such as industrial or non-research settings.
Product Description
ProSignalTM Femto ECL Reagent, Low-femtogram, 1000cm2, 100ml KitUnit
Customer Questions & Answers
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Question:
What is ProSignal Pico ECL Reagent used for?
Answer: ProSignal Pico ECL Reagent is primarily designed for enhanced chemiluminescent detection in Western blotting and other protein analysis techniques. This reagent allows researchers to visualize low-abundance proteins with high specificity and sensitivity, making it an essential tool in biochemistry and molecular biology studies. It's perfect for scenarios where detecting minute protein amounts is crucial, such as in research on disease biomarkers or protein interactions. -
Question:
How does ProSignal Pico ECL Reagent work?
Answer: The ProSignal Pico ECL Reagent functions by producing light when an enzyme-substrate reaction occurs in the presence of a specific antibody. This chemiluminescent signal is then captured on film or a digital imaging system, allowing for the visualization and quantification of proteins. Its low-picogram sensitivity allows even the faintest protein bands to be detected, which is valuable in experiments requiring precise measurements of protein expression levels. -
Question:
What are the benefits of using a low-picogram ECL reagent?
Answer: Using a low-picogram ECL reagent, like the ProSignal Pico, provides significant advantages in sensitivity and detection limits. Researchers can accurately detect and quantify proteins present in very low concentrations, which is particularly beneficial in proteomics and diagnostic applications. This heightened sensitivity can lead to more reliable results and can help in identifying disease-related proteins that would otherwise go undetected. -
Question:
Is the ProSignal Pico ECL Reagent suitable for all types of proteins?
Answer: Yes, the ProSignal Pico ECL Reagent is designed to be versatile and can be used for a wide range of proteins, including those with various post-translational modifications. Its formulation allows it to work effectively in diverse applications, whether you are analyzing antibodies, transcription factors, or signaling molecules. This versatility makes it an essential resource for any lab working with protein detection. -
Question:
What techniques can I use with the ProSignal Pico ECL Reagent?
Answer: The ProSignal Pico ECL Reagent is compatible with several techniques, primarily Western blotting, but also includes applications like dot blotting and cell lysate analysis. This compatibility makes the reagent a valuable asset in various research fields, from cell biology to immunology. For instance, researchers studying cell signaling pathways often rely on ECL reagents to detect phosphorylated proteins accurately. -
Question:
Can the ProSignal Pico ECL Reagent be used for quantitative analysis?
Answer: Absolutely, the ProSignal Pico ECL Reagent is highly suitable for quantitative analysis due to its robust signal and low background noise. By creating a standard curve with known protein concentrations, researchers can quantify unknown samples against this baseline. This quantitative capability is particularly crucial in experiments where precise measurements are required for protein levels in clinical research or drug efficacy studies. -
Question:
What is the recommended protocol for using ProSignal Pico ECL Reagent?
Answer: To effectively use the ProSignal Pico ECL Reagent, follow a detailed protocol that involves preparing your samples and running gel electrophoresis. After transferring proteins to a membrane, incubate with specific antibodies followed by the ProSignal Reagent. Incubation duration may vary, but it's essential to optimize conditions for the best signal. This procedural clarity assists researchers in achieving high-resolution results, leading to meaningful insights in their studies. -
Question:
Where can I find support for using ProSignal Pico ECL Reagent?
Answer: For support when using ProSignal Pico ECL Reagent, users can consult detailed product documentation typically available on the manufacturer's website. Many vendors also provide technical support and troubleshooting guides tailored to common issues encountered during usage. Additionally, you might find user forums and workshops helpful, as these platforms allow sharing best practices and tips among fellow researchers. -
Question:
How should I store the ProSignal Pico ECL Reagent?
Answer: Proper storage of ProSignal Pico ECL Reagent is crucial for maintaining its effectiveness. Typically, it should be stored at 4°C and protected from light to prevent degradation of sensitive components. For labs that frequently use this reagent, aliquoting into smaller volumes can help minimize freeze-thaw cycles, preserving its activity. Correct storage ensures consistent performance, allowing researchers to rely on its capabilities every time they analyze protein samples. -
Question:
Where can I buy ProSignal Pico ECL Reagent in Ireland?
Answer: You can purchase the ProSignal Pico ECL Reagent from Ubuy, a leading online platform that offers a wide range of scientific products including laboratory reagents. Ubuy provides a user-friendly interface and reliable service, making it easy for customers to find the exact product they need. Simply visit their website, search for the ProSignal Pico ECL Reagent, and add it to your cart for quick access.
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€ 573
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Ubuy works hard to protect your security and privacy. Our advanced payment security system ensures confidentiality by encrypting your information during transmission using AES (Advanced Encryption Standards) and SSL (Secure Socket Layer) protocols. Your payment details are 100% secure as we do not share your payment details with third party sellers.
Features & Benefits
- Enhanced ECL HRP substrate for Western blot analysis.
- Detects low-picogram amounts of protein with high sensitivity.
- Intense signal duration of over 6 hours post-incubation.
- Optimized for dilute antibody concentrations to save on resources.
- Allows multiple exposures without significant signal loss.
- Not intended for quantitative Western blotting.