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Item #:
141798577
EGTA [Ethylene Glycol-bis2-aminoethylether-N,N,N,N-tetraacetic Acid], 50 Grams
Item #:
141798577
€ 179
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What Stands Out
High Purity
EGTA offers superior purity, ensuring reliable results in complex biochemical applications, minimizing contamination risks when chelating essential metal ions.
Versatile Applications
Ideal for research in molecular biology, EGTA effectively stabilizes cellular systems, making it indispensable for various experimental protocols and biochemical assays.
Easy Handling
Packaged conveniently in a 50-gram format, EGTA allows for precise measurement and easy integration into experiments, catering to both novice and experienced researchers.
Product Details
| Item Weight | 0.11 lbs (50 grams) |
Dangerous Goods
To avoid the delay in customs clearance process for Dangerous Goods, the shipment shall be opened at our warehouse facility to check the severity of hazardous components. Please see the list of Dangerous goods here.
Who Should Buy?
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Biochemists
Ideal for biochemists who require a chelating agent for metal ion sequestration in enzyme activity assays.
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Molcular Biologists
Useful for molecular biologists needing specific calcium ion binding in research and experimental settings.
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Lab Researchers
Beneficial for laboratory researchers performing experiments where calcium chelation is essential to control reactions.
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General Consumers
Not suitable for everyday consumers without specialized knowledge in chemistry or biochemical applications.
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Non-Scientific Use
Inappropriate for applications outside scientific research, such as household cleaning or gardening.
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Students Without Guidance
May be confusing for students without proper laboratory guidance or supervision in using this chemical safely.
Product Description
EGTA [Ethylene Glycol-bis2-aminoethylether-N,N,N,N-tetraacetic Acid], 50 Grams
Have any Query? Chat with us
Customer Questions & Answers
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Question:
What is EGTA and what are its main uses?
Answer: EGTA, or Ethylene Glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic Acid, is a chelating agent primarily used to bind metal ions in biochemical and molecular biology applications. It is particularly effective in biological studies, as it helps maintain ion concentrations while allowing for precise experimental conditions. EGTA is commonly utilized in protein purification, enzyme assays, and cell culture applications, where controlling calcium levels is crucial. -
Question:
What are the benefits of using EGTA over other chelators?
Answer: EGTA is preferred over other chelators like EDTA in specific scenarios due to its stronger affinity for calcium ions compared to magnesium. This selectivity is vital in research where the precise manipulation of ion concentrations is required. For instance, in enzymatic reactions where calcium must be chelated without affecting magnesium dependent processes, EGTA provides enhanced specificity, making it indispensable for experiments in cell signaling and enzymology. -
Question:
How should EGTA be stored for optimal performance?
Answer: To ensure that EGTA maintains its effectiveness, it should be stored in a cool, dry place, away from direct sunlight and humidity. A tightly sealed container protects it from moisture and contaminants. Proper storage conditions prolong its shelf life and maintain its chelating properties, making it suitable for consistent use in experiments. Moreover, storing it at room temperature ensures that it remains readily available when needed. -
Question:
What concentrations of EGTA are typically used in laboratory protocols?
Answer: The concentration of EGTA used largely depends on the specific application, but common concentrations range from 1 mM to 10 mM. In cell culture experiments, for example, a lower concentration may be sufficient to bind excess calcium without disrupting the cellular environment. Conversely, more concentrated solutions may be necessary for certain biochemical assays, allowing researchers to carefully tailor conditions based on their experimental needs. -
Question:
Is EGTA safe to handle in laboratory environments?
Answer: While EGTA is generally considered safe to handle when used appropriately, it is important to follow standard laboratory safety protocols. This includes wearing gloves and eye protection, as well as working in a well-ventilated area. Although EGTA is not classified as highly hazardous, it’s always prudent to handle all chemicals with care to prevent skin or eye irritation in case of accidental exposure. -
Question:
Can EGTA be used for biological studies involving cells?
Answer: Yes, EGTA is frequently used in biological studies, especially ones that require precise control of calcium concentrations in cell systems. For instance, in cell signaling studies, EGTA can be utilized to chelate calcium ions, allowing researchers to observe cellular responses in the absence of calcium. This application is essential for understanding various cellular processes and signaling pathways. -
Question:
What are the common sources of contamination when using EGTA?
Answer: Common sources of contamination when using EGTA can include poorly cleaned glassware, exposure to atmospheric moisture, and improper handling. To minimize these risks, it’s crucial to maintain a clean working environment and use high-quality reagents. Additionally, sealing containers tightly after use helps prevent moisture from compromising the EGTA’s chelating capabilities, ensuring reliable results in your experiments. -
Question:
Does EGTA have any effects on enzyme activity?
Answer: EGTA can have significant effects on enzyme activity, particularly those that require calcium for activation. By chelating calcium, it can inhibit the activity of calcium-dependent enzymes, allowing researchers to study the enzyme's function and regulation without calcium interference. Cases in biochemical research often involve a step where enzyme inhibition is necessitated by removing calcium ions, which EGTA accomplishes effectively. -
Question:
What is the role of EGTA in protein purification?
Answer: In protein purification, EGTA plays a critical role by preventing the binding of calcium ions that may affect protein stability and function. Its use is especially crucial in chromatographic techniques, where it ensures that proteins remain in their desired conformations during purification processes. This allows for higher purity levels and better yields of proteins, making EGTA an essential reagent in biochemistry and molecular biology laboratories. -
Question:
Where can I buy EGTA [Ethylene Glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic Acid], 50 Grams?
Answer: You can purchase EGTA [Ethylene Glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic Acid], 50 Grams at Ubuy. They offer a wide selection of chemicals and laboratory supplies, ensuring you can conveniently acquire this essential reagent for your research needs.
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€ 179
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Features & Benefits
- CAS Number: 67-42-5
- Molecular Formula: C14H24N2O10
- Molecular Weight: 380.35
- Common Name: Ethylene glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic acid
- Ideal for research and manufacturing applications.
- High-quality chemical for professional use.
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