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Item #:
157497469
11AGAL0050 QA-Agarose Low Melting Point, 50 g
Item #:
157497469
€ 739
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QA-Agarose Low Melting has finer sieving characteristics for superior separation of DNA/RNA fragments.
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What Stands Out
Low Melting Point
This agarose features a low melting point, allowing for easy handling and flexibility in applications such as gel electrophoresis and DNA manipulations, catering to researchers' needs for efficient workflows.
High Purity
Manufactured with high purity standards, this agarose ensures minimal contaminants that could affect experimental results, providing researchers confidence in their data integrity and reliability.
Bulk Quantity
Offered in a convenient 50 g package, it is perfect for laboratories requiring large amounts of agarose for various experiments, delivering great value and minimizing the need for frequent reordering.
Product Details
| Item Weight | 0.11 lbs (50 grams) |
Who Should Buy?
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Molecular Biologists
Ideal for applications involving DNA analysis, such as gel electrophoresis and cloning protocols that require low melting point agarose.
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Researchers in Cryopreservation
Supports gel embedding techniques for biological samples intended for cryopreservation or other sensitive procedures.
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Advanced Genetic Studies
Useful for studies that require high resolution and purity in nucleic acid manipulation during experiments.
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General Lab Use
May not be suitable for routine applications where standard agarose suffices or where melting point isn't critical.
Product Description
11AGAL0050 QA-Agarose Low Melting Point, 50 g
Have any Query? Chat with us
Customer Questions & Answers
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Question:
What is QA-Agarose Low Melting Point used for?
Answer: QA-Agarose Low Melting Point is primarily used for gel electrophoresis in molecular biology applications. It is ideal for separating nucleic acids, such as DNA and RNA, due to its unique low melting temperature, which allows for easy recovery of the samples. This property makes it suitable for sensitive techniques such as cloning, sequencing, and PCR product analysis, enabling researchers to obtain high-purity products without significant degradation. -
Question:
How does Low Melting Point Agarose differ from standard agarose?
Answer: Low Melting Point Agarose typically has a lower gelation temperature compared to standard agarose. This allows the gel to remain in a liquefied state at lower temperatures, making it easier to manipulate and pour. As a result, this type of agarose is particularly beneficial when working with heat-sensitive samples or when needing to recover nucleic acids post-electrophoresis, as it minimizes thermal damage during the process. -
Question:
Can I use QA-Agarose for protein separation as well?
Answer: While QA-Agarose is primarily designed for nucleic acid applications, it can also be used in certain protein separation techniques. However, it is essential to ensure that the proteins of interest are compatible with the agarose matrix and that the electrophoresis conditions are optimized accordingly. Researchers may use QA-Agarose for protein applications where low temperatures help maintain protein integrity and activity. -
Question:
What is the appropriate concentration of agarose to use in gels?
Answer: The concentration of QA-Agarose used can vary depending on the size of the DNA or RNA fragments being separated. Common concentrations range from 0.5% to 2%, with lower concentrations used for larger fragments and higher concentrations for smaller ones. It is advisable to start with the recommended concentration for your application and optimize based on results to achieve the best separation and resolution. -
Question:
How do I prepare a QA-Agarose gel?
Answer: To prepare a QA-Agarose gel, first, dissolve the specified amount of agarose in buffer solution by heating until fully melted. Allow the solution to cool slightly, then pour it into a gel casting tray, inserting a comb to create wells. Once the agarose has solidified, it can be inserted into your electrophoresis apparatus and used for sample loading. Ensure the gel is in the appropriate buffer during electrophoresis for optimal results. -
Question:
Is QA-Agarose suitable for high-throughput applications?
Answer: Absolutely, QA-Agarose Low Melting Point is highly suitable for high-throughput applications, particularly in laboratories that process numerous samples simultaneously. The gel's low melting point facilitates rapid sample recovery, allowing researchers to efficiently move from electrophoresis to downstream applications such as cloning, sequencing, or further analysis, thus saving valuable time in experimental workflows. -
Question:
What are some common storage conditions for QA-Agarose?
Answer: QA-Agarose should be stored at room temperature, protected from moisture and extreme temperatures. It's advisable to keep the agarose in a cool, dark place and in its original packaging to prevent contamination and degradation. Proper storage conditions will extend the product's shelf life, ensuring optimal performance when used in molecular biology techniques. -
Question:
Can I use QA-Agarose for RNA separation?
Answer: Yes, QA-Agarose Low Melting Point is excellent for RNA separation due to its ability to maintain the integrity of RNA during gel electrophoresis. Since RNA is more susceptible to degradation than DNA, using a low melting point agarose helps in preventing damage during the gel preparation and electrophoresis process, making it ideal for studies involving RNA characterization and quantitation. -
Question:
What safety precautions should I take when using QA-Agarose?
Answer: When using QA-Agarose, standard laboratory safety precautions should be followed. This includes wearing suitable personal protective equipment (PPE), such as gloves and goggles, to prevent direct contact with the substance and any chemicals used during gel preparation. Additionally, handle heat sources with care during gel melting to avoid burns, and ensure proper waste disposal of used gels according to local regulations. -
Question:
Where can I buy 11AGAL0050 QA-Agarose Low Melting Point, 50 g?
Answer: You can purchase 11AGAL0050 QA-Agarose Low Melting Point, 50 g from Ubuy. This online retailer offers a variety of scientific products and is a reliable source for laboratory supplies, ensuring you find the necessary materials for your research and experiments without hassle.
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Features & Benefits
- Finer sieving properties compared to standard melt agarose.
- Ideal for separating DNA/RNA fragments greater than 500 bp.
- Low melting temperature allows for in-gel manipulation.
- No gel purification necessary, saving time and resources.
- High clarity results ensure reliable and precise outcomes.
- Suitable for viral plaque assays, enhancing versatility in applications.
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